| Boston | Roxbury | PK–12 | 46,094 |
| Worcester | Worcester | PK–12 | 24,778 |
| Springfield | Springfield | PK–12 | 23,670 |
| Lynn | Lynn | PK–12 | 16,364 |
| Brockton | Brockton | PK–12 | 15,280 |
| Lowell | Lowell | PK–12 | 14,457 |
| Lawrence | Lawrence | PK–12 | 13,313 |
| New Bedford | New Bedford | PK–12 | 12,505 |
| Newton | Newtonville | PK–12 | 11,632 |
| Fall River | Fall River | PK–12 | 11,089 |
| Quincy | Quincy | PK–12 | 9,769 |
| Framingham | Framingham | PK–12 | 9,124 |
| Taunton | Taunton | PK–12 | 8,311 |
| Haverhill | Haverhill | PK–12 | 7,940 |
| Everett | Everett | PK–12 | 7,307 |
| Revere | Revere | PK–12 | 7,261 |
| Cambridge | Cambridge | PK–12 | 7,027 |
| Brookline | Brookline | PK–12 | 7,023 |
| Plymouth | Plymouth | PK–12 | 6,956 |
| Chicopee | Chicopee | PK–12 | 6,755 |
| Lexington | Lexington | PK–12 | 6,748 |
| Wachusett | Jefferson | PK–12 | 6,557 |
| Methuen | Methuen | PK–12 | 6,464 |
| Malden | Malden | PK–12 | 6,354 |
| Attleboro | Attleboro | PK–12 | 6,239 |
| Leominster | Leominster | PK–12 | 6,193 |
| Arlington | Arlington | PK–12 | 6,113 |
| Chelsea | Chelsea | PK–12 | 6,094 |
| Shrewsbury | Shrewsbury | PK–12 | 5,956 |
| Waltham | Waltham | PK–12 | 5,777 |
| Peabody | Peabody | PK–12 | 5,774 |
| Bridgewater-Raynham | Bridgewater | PK–12 | 5,682 |
| Weymouth | Weymouth | PK–12 | 5,630 |
| Needham | Needham | PK–12 | 5,493 |
| Andover | Andover | PK–12 | 5,414 |
| Natick | Natick | PK–12 | 5,297 |
| Fitchburg | Fitchburg | PK–12 | 5,234 |
| Braintree | Braintree | PK–12 | 5,209 |
| Chelmsford | Chelmsford | PK–12 | 5,068 |
| Acton-Boxborough | Acton | PK–12 | 5,055 |
| Billerica | Billerica | PK–12 | 4,972 |
| Somerville | Somerville | PK–12 | 4,952 |
| Pittsfield | Pittsfield | PK–12 | 4,873 |
| Holyoke | Holyoke | PK–12 | 4,795 |
| Westfield | Westfield | PK–12 | 4,772 |
| Marlborough | Marlborough | PK–12 | 4,728 |
| Barnstable | Hyannis | PK–12 | 4,680 |
| Franklin | Franklin | PK–12 | 4,611 |
| Westford | Westford | PK–12 | 4,575 |
| Woburn | Woburn | PK–12 | 4,520 |
| Milford | Milford | PK–12 | 4,512 |
| Beverly | Beverly | PK–12 | 4,503 |
| Belmont | Belmont | PK–12 | 4,448 |
| North Andover | North Andover | PK–12 | 4,416 |
| Winchester | Winchester | PK–12 | 4,391 |
| Milton | Milton | PK–12 | 4,367 |
| Hopkinton | Hopkinton | PK–12 | 4,227 |
| Medford | Medford | PK–12 | 4,170 |
| West Springfield | West Springfield | PK–12 | 3,998 |
| Wellesley | Wellesley | PK–12 | 3,986 |
| North Attleborough | North Attleborough | PK–12 | 3,877 |
| Reading | Reading | PK–12 | 3,836 |
| Westborough | Westborough | PK–12 | 3,831 |
| Salem | Salem | PK–12 | 3,831 |
| Melrose | Melrose | PK–12 | 3,815 |
| Stoughton | Stoughton | PK–12 | 3,759 |
| Marshfield | Marshfield | PK–12 | 3,637 |
| Hingham | Hingham | PK–12 | 3,616 |
| Norwood | Norwood | PK–12 | 3,575 |
| Walpole | Walpole | PK–12 | 3,565 |
| Burlington | Burlington | PK–12 | 3,510 |
| Dracut | Dracut | PK–12 | 3,491 |
| Dudley-Charlton Reg | Dudley | PK–12 | 3,476 |
| Sharon | Sharon | PK–12 | 3,468 |
| Agawam | Feeding Hills | PK–12 | 3,461 |
| Whitman-Hanson | Whitman | PK–12 | 3,457 |
| Wakefield | Wakefield | PK–12 | 3,382 |
| Easton | North Easton | PK–12 | 3,360 |
| Canton | Canton | PK–12 | 3,314 |
| Dartmouth | Dartmouth | PK–12 | 3,297 |
| Mansfield | Mansfield | PK–12 | 3,283 |
| Danvers | Danvers | PK–12 | 3,238 |
| Tewksbury | Tewksbury | PK–12 | 3,219 |
| Grafton | Grafton | PK–12 | 3,089 |
| Dennis-Yarmouth | South Yarmouth | PK–12 | 3,054 |
| Nashoba | Bolton | PK–12 | 2,990 |
| Tec Connections Academy Commonwealth Virtual School District | East Walpole | K–12 | 2,969 |
| North Middlesex | Townsend | PK–12 | 2,914 |
| Ashland | Ashland | PK–12 | 2,909 |
| Hampden-Wilbraham | Wilbraham | PK–12 | 2,881 |
| Randolph | Randolph | PK–12 | 2,875 |
| Middleborough | Middleborough | PK–12 | 2,870 |
| Falmouth | East Falmouth | PK–12 | 2,866 |
| Watertown | Watertown | PK–12 | 2,844 |
| Westwood | Westwood | PK–12 | 2,842 |
| Gloucester | Gloucester | PK–12 | 2,838 |
| Longmeadow | Longmeadow | PK–12 | 2,764 |
| Wilmington | Wilmington | PK–12 | 2,756 |
| Scituate | Scituate | PK–12 | 2,744 |
| Wayland | Wayland | PK–12 | 2,701 |
| Duxbury | Duxbury | PK–12 | 2,694 |
| Holliston | Holliston | PK–12 | 2,689 |
| Saugus | Saugus | PK–12 | 2,675 |
| Dedham | Dedham | PK–12 | 2,668 |
| Freetown-Lakeville | Lakeville | PK–12 | 2,603 |
| Marblehead | Marblehead | PK–12 | 2,564 |
| Hanover | Hanover | PK–12 | 2,550 |
| Dighton-Rehoboth | North Dighton | PK–12 | 2,541 |
| Gardner | Gardner | PK–12 | 2,532 |
| Sudbury | Sudbury | PK–8 | 2,529 |
| Bedford | Bedford | PK–12 | 2,513 |
| Auburn | Auburn | PK–12 | 2,506 |
| Foxborough | Foxborough | PK–12 | 2,504 |
| Northampton | Northampton | PK–12 | 2,496 |
| Medfield | Medfield | PK–12 | 2,478 |
| East Longmeadow | East Longmeadow | PK–12 | 2,442 |
| Pembroke | Pembroke | PK–12 | 2,367 |
| Norton | Norton | PK–12 | 2,346 |
| Stoneham | Stoneham | PK–12 | 2,338 |
| North Reading | North Reading | PK–12 | 2,327 |
| Hudson | Hudson | PK–12 | 2,322 |
| Greater Lowell Regional Vocational Technical | Tyngsborough | 9–12 | 2,314 |
| Groton-Dunstable | Groton | PK–12 | 2,300 |
| Ashburnham-Westminster | Ashburnham | PK–12 | 2,255 |
| Pentucket | West Newbury | PK–12 | 2,238 |
| Brooke Charter School | Mattapan | K–12 | 2,216 |
| Ludlow | Ludlow | PK–12 | 2,210 |
| Norwell | Norwell | PK–12 | 2,194 |
| Lynnfield | Lynnfield | PK–12 | 2,178 |
| Medway | Medway | PK–12 | 2,158 |
| Rockland | Rockland | PK–12 | 2,158 |
| Greater New Bedford Regional Vocational Technical | New Bedford | 9–12 | 2,151 |
| Abington | Abington | PK–12 | 2,143 |
| Sandwich | East Sandwich | PK–12 | 2,118 |
| Quabbin | Barre | PK–12 | 2,111 |
| Newburyport | Newburyport | PK–12 | 2,107 |
| Swampscott | Swampscott | PK–12 | 2,094 |
| Triton | Byfield | PK–12 | 2,074 |
| Mendon-Upton | Mendon | PK–12 | 2,072 |
| Weston | Weston | PK–12 | 2,063 |
| Belchertown | Belchertown | PK–12 | 2,043 |
| Seekonk | Seekonk | PK–12 | 2,032 |
| Clinton | Clinton | PK–12 | 2,025 |
| Bellingham | Bellingham | PK–12 | 2,020 |
| East Bridgewater | East Bridgewater | PK–12 | 2,013 |
| Swansea | Swansea | PK–12 | 1,991 |
| Winthrop | Winthrop | PK–12 | 1,961 |
| Wareham | Wareham | PK–12 | 1,916 |
| Concord | Concord | PK–8 | 1,914 |
| Northbridge | Whitinsville | PK–12 | 1,838 |
| Greater Lawrence Regional Vocational Technical | Andover | 9–12 | 1,837 |
| Essex North Shore Agricultural and Technical School District | Hathorne | 9–12 | 1,830 |
| Southbridge | Southbridge | PK–12 | 1,828 |
| King Philip | Norfolk | 7–12 | 1,820 |
| Amesbury | Amesbury | PK–12 | 1,793 |
| Tantasqua | Fiskdale | 7–12 | 1,750 |
| Monomoy Regional School District | Chatham | PK–12 | 1,728 |
| Fairhaven | Fairhaven | PK–12 | 1,727 |
| Ayer Shirley School District | Ayer | PK–12 | 1,723 |
| Nantucket | Nantucket | PK–12 | 1,719 |
| Webster | Webster | PK–12 | 1,712 |
| Mystic Valley Regional Charter | Malden | K–12 | 1,698 |
| Hamilton-Wenham | Wenham | PK–12 | 1,669 |
| Silver Lake | Kingston | 7–12 | 1,661 |
| Littleton | Littleton | PK–12 | 1,655 |
| Kipp Academy Lynn Charter | Lynn | K–12 | 1,637 |
| Millbury | Millbury | PK–12 | 1,631 |
| Uxbridge | Uxbridge | PK–12 | 1,623 |
| Somerset | Somerset | PK–8 | 1,620 |
| South Hadley | South Hadley | PK–12 | 1,620 |
| Southeastern Regional Vocational Technical | South Easton | 9–12 | 1,618 |
| Athol-Royalston | Athol | PK–12 | 1,604 |
| Northborough | Southborough | K–8 | 1,567 |
| Ipswich | Ipswich | PK–12 | 1,563 |
| Lunenburg | Lunenburg | PK–12 | 1,563 |
| Central Berkshire | Dalton | PK–12 | 1,537 |
| Bourne | Bourne | PK–12 | 1,519 |
| Westport | Westport | PK–12 | 1,515 |
| Tyngsborough | Tyngsborough | PK–12 | 1,515 |
| Springfield International Charter | Springfield | K–12 | 1,511 |
| Blackstone-Millville | Blackstone | PK–12 | 1,480 |
| Masconomet | Boxford | 7–12 | 1,477 |
| Carver | Carver | PK–12 | 1,476 |
| Greater Fall River Regional Vocational Technical | Fall River | 9–12 | 1,463 |
| Foxborough Regional Charter | Foxborough | K–12 | 1,459 |
| Oxford | Oxford | PK–12 | 1,458 |
| Lincoln-Sudbury | Sudbury | 9–12 | 1,445 |
| Narragansett | Baldwinville | PK–12 | 1,439 |
| Abby Kelley Foster Charter Public | Worcester | K–12 | 1,426 |
| Montachusett Regional Vocational Technical | Fitchburg | 9–12 | 1,417 |
| Spencer-E Brookfield | Spencer | PK–12 | 1,398 |
| Cohasset | Cohasset | PK–12 | 1,397 |
| Mashpee | Mashpee | PK–12 | 1,386 |
| Northeast Metropolitan Regional Vocational Technical | Wakefield | 9–12 | 1,381 |
| Excel Academy Charter | East Boston | 5–12 | 1,379 |
| Greenfield | Greenfield | PK–12 | 1,370 |
| Easthampton | Easthampton | PK–12 | 1,368 |
| West Bridgewater | West Bridgewater | PK–12 | 1,357 |
| Holbrook | Holbrook | PK–12 | 1,350 |
| Bristol-Plymouth Regional Vocational Technical | Taunton | 9–12 | 1,335 |
| Southborough | Southborough | PK–8 | 1,310 |
| Southwick-Tolland-Granville Regional School District | Southwick | PK–12 | 1,295 |
| Leicester | Leicester | PK–12 | 1,294 |
| Sutton | Sutton | PK–12 | 1,291 |
| Whittier Regional Vocational Technical | Haverhill | 9–12 | 1,285 |
| Shawsheen Valley Regional Vocational Technical | Billerica | 9–12 | 1,282 |
| Atlantis Charter | Fall River | K–12 | 1,246 |
| Blackstone Valley Regional Vocational Technical | Upton | 9–12 | 1,243 |
| Maynard | Maynard | PK–12 | 1,217 |
| Collegiate Charter School of Lowell | Lowell | K–12 | 1,212 |
| Community Day Charter Public School | Lawrence | PK–8 | 1,200 |
| Northboro-Southboro | Southborough | 9–12 | 1,197 |
| Georgetown | Georgetown | PK–12 | 1,197 |
| Nauset | Orleans | 6–12 | 1,192 |
| Concord-Carlisle | Concord | 9–12 | 1,191 |
| Manchester Essex Regional | Manchester | PK–12 | 1,190 |
| Match Charter Public School | Boston | PK–12 | 1,189 |
| Southern Worcester County Regional Vocational School Distric | Charlton | 9–12 | 1,188 |
| Winchendon | Winchendon | PK–12 | 1,188 |
| Greater Commonwealth Virtual District | Greenfield | K–12 | 1,184 |
| Amherst-Pelham | Amherst | 7–12 | 1,182 |
| Mount Greylock | Williamstown | PK–12 | 1,177 |
| Kingston | Kingston | K–6 | 1,168 |
| Millis | Millis | PK–12 | 1,161 |
| North Adams | North Adams | PK–12 | 1,149 |
| Assabet Valley Regional Vocational Technical | Marlborough | 9–12 | 1,144 |
| Berkshire Hills | Stockbridge | PK–12 | 1,131 |
| Roxbury Preparatory Charter | Roxbury | 5–12 | 1,126 |
| Quaboag Regional | Warren | PK–12 | 1,115 |
| Douglas | Douglas | PK–12 | 1,113 |
| Berlin-Boylston | Boylston | PK–12 | 1,111 |
| Hopedale | Hopedale | PK–12 | 1,108 |
| Palmer | Palmer | PK–12 | 1,093 |
| Ware | Ware | PK–12 | 1,082 |
| Dover-Sherborn | Dover | 6–12 | 1,081 |
| South Shore Charter Public | Norwell | K–12 | 1,057 |
| Norfolk | Norfolk | PK–6 | 1,056 |
| Old Rochester | Mattapoisett | 7–12 | 1,050 |
| Alma Del Mar Charter School | New Bedford | K–8 | 1,042 |
| Lincoln | Lincoln | PK–8 | 1,039 |
| Amherst | Amherst | PK–6 | 1,038 |
| Harvard | Harvard | PK–12 | 1,023 |
| Tri-County Regional Vocational Technical | Franklin | 9–12 | 1,000 |
| Hoosac Valley Regional | Adams | PK–12 | 998 |
| Advanced Math and Science Academy Charter | Marlborough | 6–12 | 970 |
| Wrentham | Wrentham | PK–6 | 958 |
| Boston Renaissance Charter Public | Hyde Park | PK–6 | 938 |
| Hampden Charter School of Science | Chicopee | 6–12 | 938 |
| West Boylston | West Boylston | PK–12 | 923 |
| Acushnet | Acushnet | PK–8 | 920 |
| Blue Hills Regional Vocational Technical | Canton | 9–12 | 910 |
| Lawrence Family Development Charter | Lawrence | PK–8 | 899 |
| Benjamin Franklin Classical Charter Public | Franklin | K–8 | 898 |
| South Middlesex Regional Vocational Technical | Framingham | 9–12 | 896 |
| Somerset Berkley Regional School District | Somerset | 9–12 | 896 |
| Prospect Hill Academy Charter | Cambridge | PK–12 | 890 |
| Gill-Montague | Turners Falls | PK–12 | 858 |
| Sturbridge | Fiskdale | PK–6 | 842 |
| Upper Cape Cod Regional Vocational Technical | Bourne | 9–12 | 838 |
| Berkley | Berkley | PK–8 | 833 |
| Sturgis Charter Public | Hyannis | 9–12 | 825 |
| Lowell Community Charter Public | Lowell | PK–8 | 816 |
| Mohawk Trail | Shelburne Falls | PK–12 | 805 |
| Monson | Monson | PK–12 | 800 |
| Pioneer Charter School of Science | Everett | K–12 | 795 |
| Neighborhood House Charter | Dorchester | PK–12 | 786 |
| Boxford | Boxford | PK–6 | 778 |
| Lenox | Lenox | PK–12 | 771 |
| Innovation Academy Charter | Tyngsborough | 5–12 | 770 |
| Nashoba Valley Regional Vocational Technical | Westford | 9–12 | 767 |
| Martha's Vineyard | Vineyard Haven | 9–12 | 744 |
| New Heights Charter School of Brockton | Brockton | 6–12 | 734 |
| Avon | Avon | PK–12 | 732 |
| Middleton | Boxford | PK–6 | 730 |
| Hampshire | Westhampton | 7–12 | 718 |
| Veritas Preparatory Charter School | Springfield | 5–11 | 712 |
| Hull | Hull | PK–12 | 712 |
| Boston Collegiate Charter | Dorchester | 5–12 | 708 |
| Gateway | Huntington | PK–12 | 707 |
| Holyoke Community Charter | Holyoke | K–8 | 701 |
| Boston Preparatory Charter Public | Hyde Park | 6–12 | 700 |
| South Shore Regional Vocational Technical | Hanover | 9–12 | 685 |
| Cape Cod Regional Vocational Technical | Harwich | 9–12 | 682 |
| Minuteman Regional Vocational Technical | Lexington | 9–12 | 669 |
| Learning First Charter Public School | Worcester | K–8 | 666 |
| Granby | Granby | PK–12 | 665 |
| Pathfinder Regional Vocational Technical | Palmer | 9–12 | 660 |
| Lee | Lee | PK–12 | 659 |
| Plainville | Plainville | PK–6 | 655 |
| Kipp Academy Boston Charter School | Mattapan | K–8 | 646 |
| Franklin County Regional Vocational Technical | Turners Falls | 9–12 | 643 |
| Up Academy Charter School of Dorchester | Boston | PK–8 | 640 |
| Rockport | Rockport | PK–12 | 631 |
| Bristol County Agricultural | Dighton | 9–12 | 631 |
| Pioneer Charter School of Science Ii | Saugus | K–12 | 621 |
| Rising Tide Charter Public | Plymouth | 5–12 | 617 |
| Carlisle | Carlisle | PK–8 | 612 |
| Southern Berkshire | Sheffield | PK–12 | 607 |
| Topsfield | Boxford | PK–6 | 598 |
| Norfolk County Agricultural | Walpole | 9–12 | 597 |