| Laramie County School District #1 | Cheyenne | PK–12 | 13,426 |
| Natrona County School District #1 | Casper | PK–12 | 12,352 |
| Campbell County School District #1 | Gillette | PK–12 | 8,459 |
| Sweetwater County School District #1 | Rock Springs | K–12 | 4,789 |
| Albany County School District #1 | Laramie | PK–12 | 3,752 |
| Sheridan County School District #2 | Sheridan | PK–12 | 3,454 |
| Lincoln County School District #2 | Afton | PK–12 | 2,924 |
| Teton County School District #1 | Jackson | PK–12 | 2,827 |
| Uinta County School District #1 | Evanston | K–12 | 2,612 |
| Fremont County School District #25 | Riverton | PK–12 | 2,354 |
| Sweetwater County School District #2 | Green River | K–12 | 2,286 |
| Park County School District # 6 | Cody | K–12 | 2,008 |
| Park County School District # 1 | Powell | K–12 | 1,778 |
| Converse County School District #1 | Douglas | K–12 | 1,639 |
| Carbon County School District #1 | Rawlins | PK–12 | 1,633 |
| Goshen County School District #1 | Torrington | K–12 | 1,617 |
| Fremont County School District # 1 | Lander | K–12 | 1,566 |
| Big Horn County School District #1 | Cowley | PK–12 | 1,251 |
| Sheridan County School District #1 | Ranchester | K–12 | 1,241 |
| Crook County School District #1 | Sundance | K–12 | 1,203 |
| Johnson County School District #1 | Buffalo | PK–12 | 1,137 |
| Washakie County School District #1 | Worland | K–12 | 1,092 |
| Sublette County School District #1 | Pinedale | K–12 | 1,032 |
| Laramie County School District #2 | Pine Bluffs | K–12 | 1,030 |
| Niobrara County School District #1 | Lusk | PK–12 | 935 |
| Platte County School District #1 | Wheatland | PK–12 | 882 |
| Weston County School District #7 | Upton | K–12 | 859 |
| Weston County School District #1 | Newcastle | K–12 | 745 |
| Uinta County School District #6 | Lyman | K–12 | 724 |
| Big Horn County School District #2 | Lovell | PK–12 | 708 |
| Uinta County School District #4 | Mountain View | K–12 | 684 |
| Lincoln County School District #1 | Diamondville | K–12 | 637 |
| Converse County School District #2 | Glenrock | PK–12 | 634 |
| Hot Springs County School District #1 | Thermopolis | PK–12 | 584 |
| Carbon County School District #2 | Saratoga | K–12 | 573 |
| Fremont County School District #14 | Ethete | PK–12 | 573 |
| Big Horn County School District #3 | Greybull | PK–12 | 447 |
| Fremont County School District #38 | Arapahoe | PK–12 | 446 |
| Sublette County School District #9 | Big Piney | K–12 | 431 |
| Fremont County School District #21 | Ft. Washakie | PK–12 | 413 |
| Fremont County School District #24 | Shoshoni | PK–12 | 387 |
| Fremont County School District # 6 | Pavillion | PK–12 | 342 |
| Wyoming Classical Academy | Mills | K–7 | 298 |
| Big Horn County School District #4 | Basin | PK–12 | 251 |
| St. Stephens Indian School | St. Stephens | K–12 | 193 |
| Platte County School District #2 | Guernsey | K–12 | 190 |
| Fremont County School District # 2 | Dubois | PK–12 | 182 |
| Cheyenne Classical Academy | Cheyenne | K–5 | 150 |
| Washakie County School District #2 | Ten Sleep | PK–12 | 104 |
| Park County School District #16 | Meeteetse | PK–12 | 98 |
| Prairie View Community School | Chugwater | PK–12 | 90 |
| Sheridan County School District #3 | Clearmont | PK–12 | 86 |
| Boces 5 | Wilson | PK–12 | — |
| Northwest Wyoming Boces | Thermopolis | K–8 | — |
| Red Top Meadows - Administration Office | Wilson | 6–12 | — |
| Saint Joseph's Children's Home - Administration Office | Torrington | K–12 | — |
| Cathedral Home for Children - Administration Office | Laramie | 4–12 | — |
| Wyoming Department of Health | Cheyenne | PK–PK | — |
| Wyoming Behavioral Institute - Administration Office | Casper | K–12 | — |
| Rite of Passage | Cheyenne | 6–12 | — |
| Northeast Wyoming Boces | Gillette | PK–12 | — |
| Wyoming Department of Family Services | Cheyenne | 7–12 | — |
| Youth Emergency Services Inc. - Administration Office | Gillette | 7–12 | — |