| Columbus City Schools District | Columbus | PK–12 | 46,515 |
| Cincinnati Public Schools | Cincinnati | PK–12 | 34,531 |
| Cleveland Municipal | Cleveland | PK–12 | 33,143 |
| Olentangy Local | Lewis Center | PK–12 | 24,133 |
| South-Western City | Grove City | PK–12 | 21,924 |
| Toledo City | Toledo | PK–12 | 21,290 |
| Akron City | Akron | PK–12 | 19,986 |
| Lakota Local | Liberty Township | PK–12 | 17,885 |
| Dublin City | Dublin | PK–12 | 16,967 |
| Hilliard City | Columbus | PK–12 | 16,298 |
| Ohio Virtual Academy | Maumee | K–12 | 15,339 |
| Westerville City | Westerville | PK–12 | 14,589 |
| Dayton City | Dayton | PK–12 | 12,785 |
| Pickerington Local | Pickerington | PK–12 | 11,729 |
| Worthington City | Worthington | PK–12 | 10,874 |
| Mason City | Mason | PK–12 | 10,155 |
| Fairfield City | Fairfield | PK–12 | 9,591 |
| Hamilton City | Hamilton | PK–12 | 9,014 |
| Parma City | Parma | PK–12 | 9,001 |
| Gahanna-Jefferson City | Gahanna | PK–12 | 8,290 |
| Centerville City | Centerville | PK–12 | 8,264 |
| Northwest Local | Cincinnati | PK–12 | 8,260 |
| Beavercreek City | Beavercreek | PK–12 | 8,099 |
| Sylvania Schools | Sylvania | PK–12 | 7,700 |
| West Clermont Local | Cincinnati | PK–12 | 7,651 |
| Kettering City School District | Kettering | PK–12 | 7,635 |
| Canton City | Canton | PK–12 | 7,478 |
| Oak Hills Local | Cincinnati | PK–12 | 7,381 |
| Springfield City School District | Springfield | PK–12 | 7,288 |
| Reynoldsburg City | Reynoldsburg | PK–12 | 7,130 |
| Mentor Exempted Village | Mentor | PK–12 | 6,896 |
| Willoughby-Eastlake City | Eastlake | PK–12 | 6,861 |
| Forest Hills Local | Cincinnati | PK–12 | 6,827 |
| Upper Arlington City | Upper Arlington | PK–12 | 6,669 |
| Washington Local | Toledo | PK–12 | 6,663 |
| Groveport Madison Local | Groveport | PK–12 | 6,295 |
| Alternative Education Academy | Independence | K–12 | 6,276 |
| Lancaster City | Lancaster | PK–12 | 6,248 |
| Springboro Community City | Springboro | PK–12 | 6,194 |
| Lorain City | Lorain | PK–12 | 6,150 |
| Sycamore Community City | Blue Ash | PK–12 | 6,051 |
| Milford Exempted Village | Milford | PK–12 | 5,961 |
| Middletown City | Middletown | PK–12 | 5,946 |
| Brunswick City | Brunswick | PK–12 | 5,931 |
| Princeton City | Cincinnati | PK–12 | 5,927 |
| Plain Local | Canton | K–12 | 5,874 |
| Medina City Sd | Medina | PK–12 | 5,867 |
| Huber Heights City | Huber Heights | PK–12 | 5,815 |
| Newark City | Newark | PK–12 | 5,789 |
| Marysville Exempted Village | Marysville | PK–12 | 5,781 |
| Strongsville City | Strongsville | PK–12 | 5,757 |
| Jackson Local | Massillon | K–12 | 5,741 |
| Elyria City Schools | Elyria | PK–12 | 5,702 |
| Findlay City | Findlay | PK–12 | 5,694 |
| Little Miami Local | Maineville | PK–12 | 5,672 |
| Delaware City | Delaware | PK–12 | 5,650 |
| Perrysburg Exempted Village | Perrysburg | PK–12 | 5,584 |
| Berea City | Berea | PK–12 | 5,332 |
| Licking Heights Local | Pataskala | PK–12 | 5,232 |
| Lebanon City | Lebanon | PK–12 | 5,202 |
| Ohio Connections Academy Inc | Worthington | K–12 | 5,191 |
| Kings Local | Kings Mills | PK–12 | 5,073 |
| Southwest Licking Local | Pataskala | PK–12 | 5,039 |
| Cleveland Heights-University Heights City | University Heights | PK–12 | 5,019 |
| New Albany-Plain Local | New Albany | PK–12 | 4,991 |
| Stow-Munroe Falls City School District | Stow | PK–12 | 4,926 |
| Miamisburg City | Miamisburg | PK–12 | 4,865 |
| Warren City | Warren | PK–12 | 4,717 |
| Northmont City | Englewood | PK–12 | 4,676 |
| Solon City | Solon | PK–12 | 4,594 |
| Youngstown City | Youngstown | PK–12 | 4,570 |
| Teays Valley Local | Ashville | PK–12 | 4,538 |
| North Ridgeville City | North Ridgeville | PK–12 | 4,486 |
| Hudson City | Hudson | PK–12 | 4,468 |
| Euclid City | Euclid | PK–12 | 4,464 |
| Shaker Heights City | Shaker Heights | PK–12 | 4,400 |
| Lakewood City | Lakewood | PK–12 | 4,369 |
| Southwest Local | Harrison | K–12 | 4,340 |
| North Canton City | North Canton | PK–12 | 4,334 |
| Avon Local | Avon | PK–12 | 4,303 |
| Big Walnut Local | Sunbury | PK–12 | 4,298 |
| Wadsworth City | Wadsworth | PK–12 | 4,275 |
| Fairborn City | Fairborn | PK–12 | 4,265 |
| Perry Local | Massillon | PK–12 | 4,240 |
| Mayfield City | Mayfield Heights | PK–12 | 4,209 |
| Anthony Wayne Local | Whitehouse | K–12 | 4,135 |
| North Royalton City | North Royalton | PK–12 | 4,134 |
| Austintown Local Schools | Youngstown | PK–12 | 4,112 |
| Riverside Local | Painesville | PK–12 | 4,112 |
| Massillon City | Massillon | PK–12 | 4,077 |
| Loveland City | Loveland | PK–12 | 4,041 |
| Marion City | Marion | PK–12 | 4,040 |
| Winton Woods City | Cincinnati | PK–12 | 3,985 |
| Troy City | Troy | K–12 | 3,929 |
| Green Local | Uniontown | PK–12 | 3,921 |
| Twinsburg City | Twinsburg | PK–12 | 3,854 |
| Cuyahoga Falls City School District | Cuyahoga Falls | K–12 | 3,848 |
| Mad River Local | Dayton | PK–12 | 3,756 |
| Canal Winchester Local | Canal Winchester | PK–12 | 3,706 |
| Brecksville-Broadview Heights City | Brecksville | PK–12 | 3,686 |
| Xenia Community City | Xenia | PK–12 | 3,611 |
| North Olmsted City | North Olmsted | PK–12 | 3,539 |
| Amherst Exempted Village | Amherst | PK–12 | 3,533 |
| Boardman Local | Youngstown | K–12 | 3,515 |
| Olmsted Falls City | Olmsted Falls | PK–12 | 3,507 |
| Whitehall City | Whitehall | PK–12 | 3,472 |
| Barberton City | Barberton | PK–12 | 3,448 |
| Adams County Ohio Valley Local | West Union | PK–12 | 3,435 |
| Avon Lake City | Avon Lake | PK–12 | 3,435 |
| Nordonia Hills City | Northfield | K–12 | 3,405 |
| Mount Vernon City | Mount Vernon | PK–12 | 3,374 |
| Lima City | Lima | PK–12 | 3,370 |
| Logan-Hocking Local | Logan | PK–12 | 3,364 |
| Highland Local | Medina | PK–12 | 3,337 |
| Edgewood City School District | Trenton | PK–12 | 3,332 |
| Oregon City | Oregon | K–12 | 3,318 |
| Lake Local | Uniontown | K–12 | 3,301 |
| Hamilton Local | Columbus | PK–12 | 3,268 |
| Fremont City | Fremont | PK–12 | 3,247 |
| Kent City | Kent | PK–12 | 3,233 |
| Wooster City | Wooster | PK–12 | 3,222 |
| Springfield Local | Holland | PK–12 | 3,214 |
| South Euclid-Lyndhurst City | Lyndhurst | PK–12 | 3,203 |
| Westlake City | Westlake | PK–12 | 3,183 |
| West Carrollton City | West Carrollton | PK–12 | 3,170 |
| Sidney City | Sidney | PK–12 | 3,149 |
| Sandusky City | Sandusky | PK–12 | 3,105 |
| Piqua City | Piqua | K–12 | 3,069 |
| Zanesville City | Zanesville | PK–12 | 3,061 |
| Aurora City | Aurora | PK–12 | 3,049 |
| Maple Heights City | Maple Heights | PK–12 | 3,024 |
| Alliance City | Alliance | PK–12 | 3,019 |
| Ashland City | Ashland | PK–12 | 3,007 |
| Great Oaks Career Campuses | Cincinnati | 5–12 | 2,999 |
| Northeastern Local | Springfield | PK–12 | 2,961 |
| Mansfield City | Mansfield | PK–12 | 2,953 |
| Monroe Local | Monroe | PK–12 | 2,925 |
| Revere Local | Richfield | PK–12 | 2,898 |
| Bedford City | Bedford | PK–12 | 2,887 |
| Vandalia-Butler City | Vandalia | PK–12 | 2,880 |
| Talawanda City | Oxford | PK–12 | 2,875 |
| Steubenville City | Steubenville | PK–12 | 2,841 |
| New Philadelphia City | New Philadelphia | PK–12 | 2,826 |
| Celina City | Celina | PK–12 | 2,815 |
| Wapakoneta City | Wapakoneta | PK–12 | 2,786 |
| Tri-Valley Local | Dresden | PK–12 | 2,785 |
| Louisville City | Louisville | PK–12 | 2,768 |
| Painesville City Local | Painesville | PK–12 | 2,744 |
| Copley-Fairlawn City | Copley | K–12 | 2,726 |
| Bellbrook-Sugarcreek Local | Bellbrook | K–12 | 2,725 |
| Mt Healthy City | Cincinnati | PK–12 | 2,704 |
| Goshen Local | Goshen | PK–12 | 2,695 |
| Dover City | Dover | PK–12 | 2,658 |
| Ashtabula Area City | Ashtabula | PK–12 | 2,635 |
| Madison Local | Madison | PK–12 | 2,631 |
| Madison Local | Mansfield | PK–12 | 2,626 |
| Ross Local | Hamilton | PK–12 | 2,607 |
| Rocky River City | Rocky River | PK–12 | 2,606 |
| Western Brown Local | Mount Orab | K–12 | 2,604 |
| Franklin City | Franklin | K–12 | 2,602 |
| Chillicothe City | Chillicothe | PK–12 | 2,589 |
| Garfield Heights City Schools | Garfield Heights | PK–12 | 2,581 |
| Midview Local | Grafton | PK–12 | 2,574 |
| Tecumseh Local | New Carlisle | K–12 | 2,555 |
| Bowling Green City School District | Bowling Green | PK–12 | 2,537 |
| Trotwood-Madison City | Trotwood | PK–12 | 2,529 |
| Kenston Local | Chagrin Falls | PK–12 | 2,524 |
| Granville Exempted Village | Granville | K–12 | 2,517 |
| Tallmadge City | Tallmadge | K–12 | 2,507 |
| Batavia Local | Batavia | PK–12 | 2,505 |
| Miami Trace Local | Washington Court House | K–12 | 2,497 |
| Bexley City | Bexley | PK–12 | 2,496 |
| Chardon Local | Chardon | PK–12 | 2,457 |
| Norwalk City | Norwalk | PK–12 | 2,453 |
| Canfield Local | Canfield | K–12 | 2,453 |
| Tiffin City Schools | Tiffin | PK–12 | 2,420 |
| Jonathan Alder Local | Plain City | PK–12 | 2,412 |
| Defiance City | Defiance | K–12 | 2,344 |
| Bay Village City | Bay Village | PK–12 | 2,333 |
| Cloverleaf Local | Lodi | PK–12 | 2,330 |
| Tipp City Exempted Village | Tipp City | K–12 | 2,323 |
| Buckeye Valley Local | Delaware | PK–12 | 2,298 |
| Bloom-Carroll Local | Carroll | K–12 | 2,294 |
| Howland Local | Warren | PK–12 | 2,283 |
| Athens City School District | Chauncey | PK–12 | 2,267 |
| Greenville City | Greenville | PK–12 | 2,249 |
| Shawnee Local | Lima | K–12 | 2,239 |
| Jackson City | Jackson | PK–12 | 2,236 |
| Bellefontaine City | Bellefontaine | PK–12 | 2,229 |
| Indian Hill Exempted Village | Cincinnati | K–12 | 2,222 |
| Hillsboro City | Hillsboro | PK–12 | 2,218 |
| Lexington Local | Lexington | PK–12 | 2,210 |
| Norton City | Norton | PK–12 | 2,185 |
| Treca Digital Academy | Marion | K–12 | 2,165 |
| Northern Local | Thornville | K–12 | 2,162 |
| London City | London | K–12 | 2,139 |
| Circleville City | Circleville | PK–12 | 2,114 |
| Buckeye Local | Medina | PK–12 | 2,110 |
| Gallia County Local | Patriot | PK–12 | 2,103 |
| Maumee City | Maumee | K–12 | 2,099 |
| Wilmington City | Wilmington | PK–12 | 2,098 |
| Union-Scioto Local | Chillicothe | PK–12 | 2,096 |
| Marietta City | Marietta | K–12 | 2,078 |
| East Muskingum Local | New Concord | PK–12 | 2,035 |
| Licking Valley Local | Newark | PK–12 | 2,035 |
| Orange City | Cleveland | PK–12 | 2,029 |
| Elida Local | Elida | K–12 | 2,016 |
| Oakwood City | Dayton | PK–12 | 2,002 |
| Clyde-Green Springs Exempted Village | Clyde | PK–12 | 1,998 |
| Warren Local | Vincent | K–12 | 1,994 |
| Bethel Local | Tipp City | K–12 | 1,991 |
| St Marys City | Saint Marys | K–12 | 1,984 |
| Woodridge Local | Peninsula | PK–12 | 1,983 |
| West Geauga Local | Chesterland | K–12 | 1,969 |
| East Liverpool City | East Liverpool | PK–12 | 1,952 |
| Geneva Area City | Geneva | PK–12 | 1,949 |
| Kipp Columbus | Columbus | K–12 | 1,945 |
| Van Wert City | Van Wert | PK–12 | 1,943 |
| Three Rivers Local | Cleves | PK–12 | 1,938 |
| Ontario Local | Ontario | PK–12 | 1,933 |
| Canton Local | Canton | PK–12 | 1,922 |
| Field Local | Mogadore | K–12 | 1,916 |
| Salem City | Salem | K–12 | 1,913 |
| Streetsboro City | Streetsboro | PK–12 | 1,905 |
| Warrensville Heights City | Warrensville Heights | PK–12 | 1,902 |
| Fairfield Union Local | Lancaster | K–12 | 1,901 |
| Indian Creek Local | Wintersville | PK–12 | 1,899 |
| Greenfield Exempted Village | Greenfield | PK–12 | 1,888 |
| Switzerland of Ohio Local | Woodsfield | PK–12 | 1,883 |
| Wyoming City | Wyoming | K–12 | 1,882 |
| New Richmond Exempted Village | New Richmond | PK–12 | 1,878 |
| Niles City | Niles | PK–12 | 1,877 |
| Ravenna City | Ravenna | PK–12 | 1,874 |
| Perkins Local | Sandusky | PK–12 | 1,868 |
| Marlington Local | Alliance | K–12 | 1,861 |
| Gallipolis City | Gallipolis | PK–12 | 1,859 |
| Kenton City | Kenton | PK–12 | 1,851 |
| Washington Court House City | Washington Court Hou | PK–12 | 1,825 |
| River Valley Local | Caledonia | PK–12 | 1,821 |
| Eaton Community City | Eaton | K–12 | 1,805 |
| Cambridge City | Cambridge | PK–12 | 1,797 |
| Shelby City | Shelby | PK–12 | 1,794 |
| Madeira City | Cincinnati | PK–12 | 1,786 |
| Urbana City | Urbana | PK–12 | 1,779 |
| Franklin Local | Duncan Falls | PK–12 | 1,776 |
| Fostoria City | Fostoria | PK–12 | 1,775 |
| Maysville Local | Zanesville | PK–12 | 1,770 |
| Valley View Local | Germantown | PK–12 | 1,766 |
| New Lexington School District | New Lexington | PK–12 | 1,758 |
| Poland Local | Poland | PK–12 | 1,755 |
| Bryan City | Bryan | PK–12 | 1,748 |
| Springfield Local | Akron | PK–12 | 1,742 |
| Carrollton Exempted Village | Carrollton | PK–12 | 1,739 |
| Hubbard Exempted Village | Hubbard | K–12 | 1,739 |
| West Branch Local | Beloit | PK–12 | 1,735 |
| Beaver Local | East Liverpool | PK–12 | 1,724 |
| Wauseon Exempted Village | Wauseon | PK–12 | 1,717 |
| Logan Elm Local | Circleville | PK–12 | 1,716 |
| Norwood City | Norwood | PK–12 | 1,698 |
| Bellevue City | Bellevue | PK–12 | 1,694 |
| Highland Local | Marengo | PK–12 | 1,693 |
| Jefferson Area Local | Jefferson | K–12 | 1,685 |
| Northwestern Local | Springfield | PK–12 | 1,681 |
| Miami Valley Career Tech | Englewood | 6–12 | 1,676 |
| West Holmes Local | Millersburg | K–12 | 1,675 |
| Chagrin Falls Exempted Village | Chagrin Falls | PK–12 | 1,667 |
| Firelands Local | South Amherst | K–12 | 1,661 |
| Vinton County Local | Mc Arthur | PK–12 | 1,656 |
| Johnstown-Monroe Local | Johnstown | K–12 | 1,656 |
| Napoleon Area City | Napoleon | PK–12 | 1,655 |
| Clark-Shawnee Local | Springfield | PK–12 | 1,650 |
| Northridge Local | Dayton | PK–12 | 1,647 |
| Lakewood Local | Hebron | PK–12 | 1,645 |
| Liberty-Benton Local | Findlay | K–12 | 1,640 |
| Otsego Local | Bowling Green | PK–12 | 1,637 |
| Northwest Local | Canal Fulton | K–12 | 1,636 |
| West Muskingum Local | Zanesville | PK–12 | 1,636 |
| Clinton-Massie Local | Clarksville | PK–12 | 1,635 |
| Buckeye Local | Ashtabula | K–12 | 1,634 |
| Minerva Local | Minerva | K–12 | 1,626 |
| St Clairsville-Richland City | Saint Clairsville | PK–12 | 1,619 |
| Great River Connections Academy | Columbus | K–12 | 1,608 |
| Waverly City | Waverly | PK–12 | 1,607 |
| Vermilion Local | Vermilion | PK–12 | 1,602 |
| Morgan Local | Mc Connelsville | PK–12 | 1,601 |
| Wheelersburg Local | Wheelersburg | PK–12 | 1,591 |
| Benjamin Logan Local | Bellefontaine | K–12 | 1,591 |
| Struthers City | Struthers | PK–12 | 1,589 |
| Indian Valley Local | Gnadenhutten | K–12 | 1,589 |
| Bath Local | Lima | PK–12 | 1,588 |
| Graham Local | Saint Paris | PK–12 | 1,588 |
| Conneaut Area City | Conneaut | PK–12 | 1,587 |
| Meigs Local | Pomeroy | PK–12 | 1,586 |
| Beachwood City | Beachwood | PK–12 | 1,586 |
| Wayne Local | Waynesville | PK–12 | 1,576 |
| Lake Local | Millbury | PK–12 | 1,569 |
| Portsmouth City | Portsmouth | PK–12 | 1,561 |
| Heath City | Heath | K–12 | 1,560 |
| Upper Sandusky Exempted Village | Upper Sandusky | K–12 | 1,560 |
| Rossford Exempted Village | Rossford | PK–12 | 1,560 |